pCS2-SP-FKBP-mCherry-TM-V5
(Plasmid
#186226)
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PurposemRNA synthesis of rapalog-induced cell adhesion molecule
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Depositing Lab
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Depositing OrganizationRIKEN Center for Biosystems Dynamics Research, Kobe
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186226 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 186226-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 186226-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2+
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSP-FKBP-mCherry-TM-V5
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SpeciesSynthetic
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Insert Size (bp)1317
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Tag
/ Fusion Protein
- V5 (C terminal on insert)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer ATTTAGGTGACACTATAGA
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 186226-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 186226-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCS2-SP-FKBP-mCherry-TM-V5 was a gift from Yoshihiro Morishita (Addgene plasmid # 186226 ; http://n2t.net/addgene:186226 ; RRID:Addgene_186226) -
For your References section:
Rapalog-induced cell adhesion molecule inhibits mesoderm migration in Xenopus embryos by increasing frequency of adhesion to the ectoderm. Usami C, Inomata H. Genes Cells. 2022 Jun;27(6):436-450. doi: 10.1111/gtc.12937. Epub 2022 May 4. 10.1111/gtc.12937 PubMed 35437867
