pAP14
(Plasmid #186262)

• Purpose: wgaA (a.k.a expA1) and wgaB (a.k.a expA23), bicistronic, under light light responsive PEL222 promoter and EL222 under constitutively active LacIq promoter
• Depositing Lab: Yongku Cho
• Publication: Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-356. doi: 10.1021/acssynbio.0c00498. Epub 2021 Jan 19.

Backbone

• Vector backbone: pSEVA531
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: wgaAB bicistronic
• Alt name: RmExpA1; RmExpA23
• Species: Synthetic
• Insert Size (bp): 4120
• GenBank ID: CAC49295.1 CAC49296.1
• Promoter: PEL222 (GGTAGCCTTTAGTCCATGTTAGCGAAGAAAATGGTTTGTTATAGTCGAATAAA)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGAAAGGCCCAGTCTTTC
• 3′ sequencing primer: CGCCAGGGTTTTCCCAGTCACGAC

Gene/Insert 2

• Gene/Insert name: EL222
• Alt name: ELI_04755
• Alt name: Light-activated DNA-binding protein EL222 from Erythrobacter litoralis
• Species: Erythrobacter litoralis
• GenBank ID: ELI_04755 ELI_04755
• Entrez Gene: ELI_04755 (a.k.a. ELI_04755)
• Promoter: LacIq (CGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAA)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATTTGACAGCTTATCATCGATAAA
• 3′ sequencing primer: TGCCGAGGATGACGATG

Resource Information

• Supplemental Documents:
  • pAP14.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry