CIRC
(Plasmid #186343)

• Purpose: Contains VEGFA exon 7 and part of intronic flanking regions enabling expression of hsa_circ_0076611 (circVEGF)
• Depositing Lab: Giovanni Blandino
• Publication: Turco et al Commun Biol. 2022 Jun 16;5(1):598. doi: 10.1038/s42003-022-03539-x.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 6177
• Modifications to backbone: Hind III to Xho I removal from MCS during cloning
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VEGFA
• Alt name: VEGF
• Species: H. sapiens (human)
• Insert Size (bp): 749
• GenBank ID: NM_001025366.3 NM_001025366.3
• Entrez Gene: VEGFA (a.k.a. L-VEGF, MVCD1, VEGF, VPF)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: HindIII (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry