pBXNPHM3-Nb_MsbA#1
(Plasmid #186428)

• Purpose: Plasmid for bacterial expression of MsbA binding Nanobody Nb_MsbA#1
• Depositing Lab: Markus Seeger
• Publication: Galazzo et al Sci Adv. 2022 Oct 14;8(41):eabn6845. doi: 10.1126/sciadv.abn6845. Epub 2022 Oct 12.

Backbone

• Vector backbone: pBXNPHM3
• Backbone size w/o insert (bp): 5311
• Total vector size (bp): 5662
• Vector type: Affinity Reagent/ Antibody

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Nanobody Nb_MsbA#1
• Species: Vicugna pacos
• Insert Size (bp): 351
• Promoter: pBAD
• Tags / Fusion Proteins:
  • PelB leader sequence (N terminal on backbone)
  • His Tag (N terminal on backbone)
  • Maltose Binding Protein (N terminal on backbone)
  • 3C cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BspQI (not destroyed)
• 3′ cloning site: BspQI (not destroyed)
• 5′ sequencing primer: ATGCCATAGCATTTTTATCC
• 3′ sequencing primer: GATTTAATCTGTATCAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry