Pet15b-Endosomal Escape Control
(Plasmid
#186552)
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PurposeNegative Control confirming the need for a CPP peptide in mediating internalization
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 186552 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonePet15b
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Backbone manufacturerNovagen
- Backbone size w/o insert (bp) 5708
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name6H-ETA-GFP
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Alt nameExotoxin A
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SpeciesBacteria
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Insert Size (bp)1225
- Promoter T7
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Tags
/ Fusion Proteins
- 6xHis (N terminal on insert)
- EGFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7 Promoter
- 3′ sequencing primer T7 Terminator (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Pet15b-Endosomal Escape Control was a gift from Jean Gariepy (Addgene plasmid # 186552 ; http://n2t.net/addgene:186552 ; RRID:Addgene_186552) -
For your References section:
The Pseudomonas aeruginosa exotoxin A translocation domain facilitates the routing of CPP-protein cargos to the cytosol of eukaryotic cells. Mohammed AF, Abdul-Wahid A, Huang EH, Bolewska-Pedyczak E, Cydzik M, Broad AE, Gariepy J. J Control Release. 2012 Nov 28;164(1):58-64. doi: 10.1016/j.jconrel.2012.10.006. Epub 2012 Oct 14. 10.1016/j.jconrel.2012.10.006 PubMed 23075769
