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paGFP-HRas G12V
(Plasmid #18694)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 18694 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    H-Ras G12V
  • Alt name
    HRas G12V
  • Alt name
    HRas
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    600
  • Mutation
    A206K mutation in paGFP G12V mutation in HRas
  • Entrez Gene
    HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, KRAS, KRAS2, Ki-Ras, RASH1, RASK2, c-K-ras, c-Ki-ras, p21ras)
  • Tag / Fusion Protein
    • photoactivatable GFP (N terminal on insert)

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    paGFP-HRas G12V was a gift from Karel Svoboda (Addgene plasmid # 18694 ; http://n2t.net/addgene:18694 ; RRID:Addgene_18694)