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pcDNA3.1-iRFP-P2A-Post-SPHERE-sfVenus-iGluSnFR
(Plasmid #187098)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 187098 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 187098-D50 50 μg of DNA in Tris buffer $495
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DNA 187098-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.1(+)
  • Backbone size w/o insert (bp) 5360
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Post-SPHERE-sfVenus-iGluSnFR
  • Species
    Synthetic
  • Insert Size (bp)
    3081
  • Promoter CMV
  • Tag / Fusion Protein
    • iRFP670-P2A (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (unknown if destroyed)
  • 3′ cloning site XbaI (unknown if destroyed)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Loren Looger, Robert Campbell and Bong-Kiun Kaang, Addgene #106202, #107337, #107335, #111585

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-iRFP-P2A-Post-SPHERE-sfVenus-iGluSnFR was a gift from Kotaro Oka (Addgene plasmid # 187098 ; http://n2t.net/addgene:187098 ; RRID:Addgene_187098)

  • For your References section:

    Genetically encoded sensors for analysing neurotransmission among synaptically-connected neurons. Shindo Y, Ashida K, Masamoto K, Takuwa H, Takahashi M, Higuchi M, Ide R, Hotta K, Oka K. bioRxiv 2022.04.03.486903; doi: https://doi.org/10.1101/2022.04.03.486903 10.1101/2022.04.03.486903
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