pLZRS-panTRK-CR1-EGFP
(Plasmid #187194)

• Purpose: To express a hybrid Trk receptor tagged with EGFP. This Hybrid TRK receptor was designed to bind NGF, BDNF and NT-3
• Depositing Lab: Pantelis Tsoulfas
• Publication: O'Connell et al J Biol Chem. 2000 Mar 17;275(11):7870-7. doi: 10.1074/jbc.275.11.7870.

Backbone

• Vector backbone: pLZRS
• Total vector size (bp): 14774
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: CR1 pan-TRK receptor
• Alt name: CR213
• Species: H. sapiens (human)
• Insert Size (bp): 3128
• Promoter: mo-MLV or MMLV
• Tag / Fusion Protein:
  • EGFP (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NotI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: pBMN 5'
• 3′ sequencing primer: EGFP-C

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Restriction digest confirmed the correct plasmid length (14,647 bp) with EBNA1 region present.