pLL5.0-EGFP-HRasC20
(Plasmid #187252)

• Purpose: Lenticiral expression of EGFP-HRasC20
• Depositing Lab: Alpha Yap
• Publication: Katsuno-Kambe et al Elife. 2021 Oct 18;10. pii: 67915. doi: 10.7554/eLife.67915.

Backbone

• Vector backbone: pLL5.0
• Backbone size w/o insert (bp): 6919
• Vector type: Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HRasC20
• Alt name: CAAX
• Species: H. sapiens (human)
• Insert Size (bp): 795
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)
• Promoter: 5' LTR
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: EGFP, forward primer

Resource Information

• Supplemental Documents:
  • pLL5.0-EGFP-HRasC20.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid made by Hiroko Kambe

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

pLL5.0-EGFP was digested with EcoRI and SbfI to remove EGFP, and then EGFP-C20 fragment was inserted and ligated by In-Fusion cloning kit.

5' cloning site: EcoR1 (destroyed), 3' cloning site: Sbf1 (destroyed).