GFP-Rock1-GBD (840-110 aa) in pEGFPN1
(Plasmid #187279)

• Purpose: Express EGFP-Rock1 GTPase-binding domain
• Depositing Lab: Alpha Yap
• Publication: Budnar et al Dev Cell. 2019 Jun 17;49(6):894-906.e12. doi: 10.1016/j.devcel.2019.04.031. Epub 2019 May 16.

Backbone

• Vector backbone: pEGFP-C1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4693
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Rock1
• Species: M. musculus (mouse)
• Insert Size (bp): 388
• Mutation: GBD (84-110aa)
• Entrez Gene: Rock1 (a.k.a. 1110055K06Rik, Rock-I)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xho1 (not destroyed)
• 3′ cloning site: BamH1 (not destroyed)
• 5′ sequencing primer: EGFP, forward primer
• 3′ sequencing primer: SV40pA-R

Resource Information

• Supplemental Documents:
  • GFP-Rock1-GBD (840-110 aa) in pEGFPN1.gb
• A portion of this plasmid was derived from a plasmid made by: Plasmid made by Srikanth Budnar

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry