pEcoFus
(Plasmid
#187833)
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Purpose(Empty Backbone) 1st generation E. coli - Fusobacterium nucleatum shuttle vector for overexpression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187833 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRPF185
- Backbone size (bp) 9035
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Modifications to backboneReplacement of origin of replication for Clostridium difficile with that for Fusobacterium nucleatum. Replacement of tetR-gus cassette with constitutive fusobacterial promoter (4.5S RNA)
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe fusobacterial origin of replication was transferred from pROI92 provided by G. Bachrach (PMID: 15574887)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEcoFus was a gift from Jörg Vogel (Addgene plasmid # 187833 ; http://n2t.net/addgene:187833 ; RRID:Addgene_187833) -
For your References section:
RNA landscape of the emerging cancer-associated microbe Fusobacterium nucleatum. Ponath F, Tawk C, Zhu Y, Barquist L, Faber F, Vogel J. Nat Microbiol. 2021 Aug;6(8):1007-1020. doi: 10.1038/s41564-021-00927-7. Epub 2021 Jul 8. 10.1038/s41564-021-00927-7 PubMed 34239075
Map uploaded by the depositor.
