HP1-ECFP-SNAP
(Plasmid #187966)

• Purpose: expresses HP1 with C-terminal tags ECFP and SNAP
• Depositing Lab: Markus Wahl
• Publication: Vester et al Commun Biol. 2022 Jul 22;5(1):736. doi: 10.1038/s42003-022-03689-y.

Backbone

• Vector backbone: pECFP-SNAP
• Backbone manufacturer: Labor Prof. Carsten Schultz, EMBL Heidelberg
• Backbone size w/o insert (bp): 6601
• Total vector size (bp): 7169
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Pir1
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HP1
• Alt name: CBX5, HP1A, HEL25
• Species: H. sapiens (human)
• Insert Size (bp): 568
• GenBank ID: NM_012117.3 NM_012117.3
• Entrez Gene: CBX5 (a.k.a. HEL25, HP1, HP1A, HP1alpha)
• Promoter: CMV
• Tag / Fusion Protein:
  • ECFP-SNAP (C terminal on insert)

Cloning Information

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: CMV-for (Microsynth)
• 3′ sequencing primer: EGFP-N-rev (Microsynth)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: ordered from Addgene No17652

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry