NSUN2
(Plasmid #188059)

• Purpose: Bacterial expression plasmid for human NSUN2; for activity testing
• Depositing Lab: Tanja Schirmeister
• Publication: Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/acs.jmedchem.2c00388.

Backbone

• Vector backbone: pET-28a(+)
• Total vector size (bp): 7642
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: NSUN2
• Alt name: RNA cytosine C(5)-methyltransferase NSUN2
• Alt name: NOP2/Sun RNA methyltransferase 2
• Species: H. sapiens (human)
• Insert Size (bp): 2313
• GenBank ID: 54888
• Entrez Gene: NSUN2 (a.k.a. MISU, MRT5, SAKI, TRM4)
• Promoter: T7 promoter
• Tags / Fusion Proteins:
  • His-tag (N terminal on insert)
  • His-tag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: T7 primer
• 3′ sequencing primer: T7 terminator reverse primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry