Chx10 (164bp crit reg+SV40 bas prom)-GFP-IRES-AP
(Plasmid
#18808)
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Depositing Lab
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Depositing OrganizationHarvard Medical School
Located in United States of America -
Publication
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Citations
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 18808 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 18808-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 18808-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAGEN
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameChx10 (164bp crit reg+SV40 bas prom)-GFP-IRES-AP
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
also known as 726341_ggia_5, Kim, D.S., Matsuda, T., and Cepko, C.L. (2008) A core paired-type and POU homeodomain-containing transcription factor program drives retinal bipolar cell gene expression. J. Neurosci. In press.
DNA (Catalog # 18808-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 18808-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Chx10 (164bp crit reg+SV40 bas prom)-GFP-IRES-AP was a gift from Connie Cepko (Addgene plasmid # 18808 ; http://n2t.net/addgene:18808 ; RRID:Addgene_18808) -
For your References section:
A core paired-type and POU homeodomain-containing transcription factor program drives retinal bipolar cell gene expression. Kim DS, Matsuda T, Cepko CL. J Neurosci. 2008 Jul 30. 28(31):7748-64. 10.1523/JNEUROSCI.0397-08.2008 PubMed 18667607
Map uploaded by the depositor.
