pGL4.23-Fosprom
(Plasmid
#188113)
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PurposeExpresses luciferase under control of Fos promoter in transfected cells
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Depositing Lab
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Depositing OrganizationUniversity of Toronto
Located in Canada -
Citations
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188113 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 188113-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 188113-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepGL4.23
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 4283
- Total vector size (bp) 5091
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Modifications to backboneMinimal promoter removed and replaced with endogenous gene promoter.
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Vector typeLuciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameFos promoter
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Alt namecFos
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SpeciesM. musculus (mouse)
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Insert Size (bp)901
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Entrez GeneFos (a.k.a. D12Rfj1, c-fos, cFos)
- Promoter Fos endogenous promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site NcoI (not destroyed)
- 5′ sequencing primer ACTTATTTACAATCCTTCACTTGCT
- 3′ sequencing primer GGTCGAAGTTTGGGGAAAGCC
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Cloning by Virlana M Shchuka.
DNA (Catalog # 188113-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 188113-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL4.23-Fosprom was a gift from Jennifer Mitchell (Addgene plasmid # 188113 ; http://n2t.net/addgene:188113 ; RRID:Addgene_188113) -
For your References section:
MYB and ELF3 differentially modulate labor-inducing gene expression in myometrial cells. Shchuka VM, Khader N, Dorogin A, Shynlova O, Mitchell JA. PLoS One. 2023 Jan 3;18(1):e0271081. doi: 10.1371/journal.pone.0271081. eCollection 2023. 10.1371/journal.pone.0271081 PubMed 36595497
