pN006
(Plasmid
#188470)
-
PurposeLentiviral construct encoding doxycycline-inducible AsCas12a (via rtTA3)
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188470 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepY108 (gift from Dr. Feng Zhang lab)
-
Vector typeMammalian Expression, Bacterial Expression, Lentiviral
-
Selectable markersPuromycin
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameAsCas12a
-
SpeciesAcidaminococcus sp.
-
Insert Size (bp)3918
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pN006 was a gift from Le Cong (Addgene plasmid # 188470 ; http://n2t.net/addgene:188470 ; RRID:Addgene_188470) -
For your References section:
Machine-learning-optimized Cas12a barcoding enables the recovery of single-cell lineages and transcriptional profiles. Hughes NW, Qu Y, Zhang J, Tang W, Pierce J, Wang C, Agrawal A, Morri M, Neff N, Winslow MM, Wang M, Cong L. Mol Cell. 2022 Jun 22. pii: S1097-2765(22)00537-8. doi: 10.1016/j.molcel.2022.06.001. 10.1016/j.molcel.2022.06.001 PubMed 35752172
Map uploaded by the depositor.
