pSC5-toxic3
(Plasmid
#188625)
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PurposepSC5GGv2 with HindII Gene Insert
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188625 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepTA-Mob 2.0
- Backbone size w/o insert (bp) 57000
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Modifications to backboneDeletion: pTA-Mob 2.0 ΔIstA-TraB Insertion: +HindII Plasmid Size: 57346 bp
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Vector typeBacterial Expression, Yeast Expression, Synthetic Biology ; Diatom Expression
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Selectable markersHIS3 ; Nourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)EPI300 (Epicentre)
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameHindII
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SpeciesHemophilus influenzae
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Insert Size (bp)3905
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MutationACT1 intron insert
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSC5-toxic3 was a gift from Bogumil Karas (Addgene plasmid # 188625 ; http://n2t.net/addgene:188625 ; RRID:Addgene_188625) -
For your References section:
Superior Conjugative Plasmids Delivered by Bacteria to Diverse Fungi. Cochrane RR, Shrestha A, Severo de Almeida MM, Agyare-Tabbi M, Brumwell SL, Hamadache S, Meaney JS, Nucifora DP, Say HH, Sharma J, Soltysiak MPM, Tong C, Van Belois K, Walker EJL, Lachance MA, Gloor GB, Edgell DR, Shapiro RS, Karas BJ. Biodes Res. 2022 Aug 19;2022:9802168. doi: 10.34133/2022/9802168. eCollection 2022. 10.34133/2022/9802168 PubMed 37850145
Map uploaded by the depositor.
