pmU6 ST
(Plasmid
#188713)
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PurposesgRNA plasmid encoding mU6 promoter driving an anti-safe targeting sgRNA
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Depositing Lab
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Depositing OrganizationDuke University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188713 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 188713-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 188713-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepmU6
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Vector typeCRISPR, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSafe Targeting sgRNA
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gRNA/shRNA sequenceGCTAAGTACTCTAACAGG
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SpeciesSynthetic
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BbsI (destroyed during cloning)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 188713-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 188713-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pmU6 ST was a gift from David Tobin (Addgene plasmid # 188713 ; http://n2t.net/addgene:188713 ; RRID:Addgene_188713) -
For your References section:
Macrophage NFATC2 mediates angiogenic signaling during mycobacterial infection. Brewer WJ, Xet-Mull AM, Yu A, Sweeney MI, Walton EM, Tobin DM. Cell Rep. 2022 Dec 13;41(11):111817. doi: 10.1016/j.celrep.2022.111817. 10.1016/j.celrep.2022.111817 PubMed 36516756
