|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18889||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 6449
Vector typeYeast Expression
Growth in Bacteria
SpeciesS. cerevisiae (budding yeast)
Entrez GeneFAR1 (a.k.a. YJL157C)
- Cloning method Restriction Enzyme
- 5′ sequencing primer pJG4_5_fwd
- 3′ sequencing primer pJG4_5_rev (Common Sequencing Primers)
Terms and Licenses
A vector like pJG4-5 with the BamHI site in reading frame CCA TGG by inserting EcoRI-SalI of the pUC19 polylinker
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pYBS135 was a gift from Elaine Elion (Addgene plasmid # 18889 ; http://n2t.net/addgene:18889 ; RRID:Addgene_18889)
For your References section:Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Choi KY, Satterberg B, Lyons DM, Elion EA. Cell. 1994 Aug 12. 78(3):499-512. 10.1016/0092-8674(94)90427-8 PubMed 8062390