pEF mito-cGFP (matrix)
(Plasmid
#188904)
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PurposeExpresses mitochondrial GFP
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Depositing Lab
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Depositing OrganizationUniversity of Wisconsin, Madison
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 188904 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 188904-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 188904-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepEF
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemitochondrial GFP
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SpeciesSynthetic
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Tag
/ Fusion Protein
- Mitochondrial leader sequence: MSVLTPLLLRGLTGSARRLPVPRAKIHSLGDPPVAT (N terminal on insert)
Cloning Information
- Cloning method Unknown
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2022.07.06.499030 for bioRxiv preprint.
DNA (Catalog # 188904-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 188904-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEF mito-cGFP (matrix) was a gift from Edwin Chapman (Addgene plasmid # 188904 ; http://n2t.net/addgene:188904 ; RRID:Addgene_188904) -
For your References section:
Mitofusin 2 Sustains the Axonal Mitochondrial Network to Support Presynaptic Ca(2+) Homeostasis and the Synaptic Vesicle Cycle in Rat Hippocampal Axons. Vevea JD, Chapman ER. J Neurosci. 2023 May 10;43(19):3421-3438. doi: 10.1523/JNEUROSCI.1356-22.2023. Epub 2023 Mar 30. 10.1523/JNEUROSCI.1356-22.2023 PubMed 36997314
Map uploaded by the depositor.
