pET3a-Ub-D77
(Plasmid #18896)

• Depositing Lab: Cecile Pickart
• Publication: Raasi et al Methods Mol Biol. 2005 . 301():47-55.

Backbone

• Vector backbone: pET3a
• Backbone size w/o insert (bp): 4600
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: BL21pJY2
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Ubiquitin-D77
• Alt name: UBC
• Species: H. sapiens (human)
• Mutation: Aspartate residue is added to the C-terminus of the insert
• Entrez Gene: UBC (a.k.a. HMG20)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Piotrowski J. has constructed this plasmid.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Piotrowski J, Beal R, Hoffman L, Wilkinson KD, Cohen RE, Pickart CM. J Biol Chem. 1997 Sep 19;272(38):23712-21.