|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||18896||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4600
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
SpeciesH. sapiens (human)
MutationAspartate residue is added to the C-terminus of the insert
Entrez GeneUBC (a.k.a. HMG20)
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (unknown if destroyed)
- 3′ cloning site BamHI (unknown if destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byPiotrowski J. has constructed this plasmid.
Terms and Licenses
- Not Available to Industry
Piotrowski J, Beal R, Hoffman L, Wilkinson KD, Cohen RE, Pickart CM. J Biol Chem. 1997 Sep 19;272(38):23712-21.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pET3a-Ub-D77 was a gift from Cecile Pickart (Addgene plasmid # 18896 ; http://n2t.net/addgene:18896 ; RRID:Addgene_18896)
For your References section:Ubiquitin chain synthesis. Raasi S, Pickart CM. Methods Mol Biol. 2005 . 301():47-55. 10.1385/1-59259-895-1:047 PubMed 15917625