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sg_ECK3180_52as
(Bacterial strain #189344)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Bacterial Strain 189344 Bacteria in agar stab 1 $94

Backbone

  • Vector backbone
    n/a
  • Vector type
    This is a strain, not a plasmid

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    BW25113
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    sg_ECK3180_52as
  • Species
    n/a

Cloning Information

  • Cloning method Unknown

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

sgRNA sequence and target gene can be found in reference: https://journals.asm.org/doi/10.1128/mBio.02561-21, Table S1 (mbio.02561-21-st001.xlsx)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    sg_ECK3180_52as was a gift from Carol Gross (Addgene plasmid # 189344)

  • For your References section:

    Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli. Silvis MR, Rajendram M, Shi H, Osadnik H, Gray AN, Cesar S, Peters JM, Hearne CC, Kumar P, Todor H, Huang KC, Gross CA. mBio. 2021 Oct 26;12(5):e0256121. doi: 10.1128/mBio.02561-21. Epub 2021 Oct 12. 10.1128/mBio.02561-21 PubMed 34634934
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