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pSCM167.2-Venus-VF-hADAM10(aa1-213)
(Plasmid #189554)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 189554 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSCM167.2-Venus (Fu lab)
  • Backbone manufacturer
    Customized backbone from Fu lab
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    truncated ADAM metallopeptidase domain 10
  • Alt name
    ADAM10; RAK; kuz; AD10; AD18; MADM; CD156c; CDw156; HsT18717
  • Species
    H. sapiens (human)
  • Mutation
    contains aa 1-213
  • Entrez Gene
    ADAM10 (a.k.a. AD10, AD18, CD156c, CDw156, HsT18717, MADM, RAK, kuz)
  • Tag / Fusion Protein
    • VF (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note: Plasmid contains L60S, D96G, and S115G mutations in hADAM10 compared to the NCBI reference sequence NP_001101.1. These mutations were present in the original DNA that was was PCR amplified from human placenta cDNA and are not known to affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSCM167.2-Venus-VF-hADAM10(aa1-213) was a gift from Haian Fu (Addgene plasmid # 189554 ; http://n2t.net/addgene:189554 ; RRID:Addgene_189554)