pAAV-TRE-HTR6-SNAPf-TRIP
(Plasmid #189617)

• Purpose: Expresses cilia-targeted RhoA inhibitory peptide, tet-inducible
• Depositing Lab: David Clapham
• Publication: Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18. doi: 10.1016/j.cell.2022.07.026.

Backbone

• Vector backbone: pAAV_TRE
• Backbone manufacturer: n/A
• Total vector size (bp): 6240
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HTR6-TRIP_alpha
• Species: H. sapiens (human)
• GenBank ID: NM_000871.3
• Promoter: TRE
• Tag / Fusion Protein:
  • alphaTag in the N-term of HTR6, SNAP-tag in the C-term of HTR6 (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: N/A

Resource Information

• Supplemental Documents:
  • 3xggggs_tripalpha_e32g_aav-tre-alphatag-h5ht6-snap_tag.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry