pFRT-PonA-BI-Gl-GFP-PTC-mCherry-WT (5FP-WP)
(Plasmid #189795)

• Purpose: Express GFP/mCherry linked wild-type and PTC-containing beta-globin bi-directionally under ponasteron A inducible promoter in mammalian cells
• Depositing Lab: Robert Singer
• Publication: Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi: 10.1038/s41467-021-27423-0.

Backbone

• Vector backbone: pFRT
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: beta-globin
• Alt name: HBB
• Species: H. sapiens (human), M. musculus (mouse)
• Entrez Gene: HBB (a.k.a. CD113t-C, ECYT6, beta-globin)
• Promoter: Ponasterone A inducible

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CGAGCTGTACAAGtaccgatgcg
• 3′ sequencing primer: ATTATGATCactagtcgtatgg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry