CAG78085
(Bacterial strain
#189937)
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PurposedCas9 donor strain, required to construct CRISPRi strains by conjugation of CAG78085 and sgRNA strains. Not available as individual bacterial stab but can be requested as part of Kit #1000000285.
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Depositing Lab
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Depositing OrganizationUniversity of California, San Francisco (UCSF)
Located in the United States of America -
Sequence Information
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Full plasmid sequence is not available for this item.
No maps are available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Bacterial Strain | 189937 | Bacteria in agar stab | 1 | $94 | |
Backbone
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Vector backbonen/a
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Vector typeThis is a strain, not a plasmid
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, Spectinomycin
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Growth Temperature37°C
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Growth Strain(s)BW25113
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCIP16 Hfr, Tn7att::Tn7C59 [dcas9]
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CAG78085 was a gift from Carol Gross (Addgene plasmid # 189937) -
For your References section:
Morphological and Transcriptional Responses to CRISPRi Knockdown of Essential Genes in Escherichia coli. Silvis MR, Rajendram M, Shi H, Osadnik H, Gray AN, Cesar S, Peters JM, Hearne CC, Kumar P, Todor H, Huang KC, Gross CA. mBio. 2021 Oct 26;12(5):e0256121. doi: 10.1128/mBio.02561-21. Epub 2021 Oct 12. 10.1128/mBio.02561-21 PubMed 34634934