H6Gb1-CITED2(216-246)+CBP TAZ1(340-439)
(Plasmid #189962)

• Purpose: Expression of CBP TAZ1 and CITED2 in E coli
• Depositing Lab: Peter Wright
• Publication: Berlow et al Proc Natl Acad Sci U S A. 2022 Jan 18;119(3). pii: 2117338119. doi: 10.1073/pnas.2117338119.

Backbone

• Vector backbone: pET21a
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5500
• Total vector size (bp): 6300
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: CITED2 (216-246)
• Species: H. sapiens (human)
• Insert Size (bp): 165
• Entrez Gene: CITED2 (a.k.a. ASD8, MRG-1, MRG1, P35SRJ, VSD2)
• Promoter: t7

Cloning Information for Gene/Insert 1

• Cloning method: Restriction Enzyme
• 5′ cloning site: NdeI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: t7
• 3′ sequencing primer: t7

Gene/Insert 2

• Gene/Insert name: CBP TAZ1 (340-439)
• Alt name: CBP CH1
• Species: M. musculus (mouse)
• Entrez Gene: Crebbp (a.k.a. CBP, CBP/p300, KAT3A, p300/CBP)
• Promoter: t7

Cloning Information for Gene/Insert 2

• Cloning method: Restriction Enzyme
• 5′ cloning site: NcoI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: t7
• 3′ sequencing primer: t7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry