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(Plasmid #190002)


Item Catalog # Description Quantity Price (USD)
Plasmid 190002 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pBA310 (Derivative of pDEX777)
  • Backbone size (bp) 6429
  • Vector type
    Expression vector for Trypanosoma brucei
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This pBA310 is a modified version of the pDEX777 plasmid (Poon et al. 2012 Open Biology and was originally published in Nerusheva and Akiyoshi 2016 Open Biology (

NLS and several restriction enzyme sites were inserted between XbaI and BamHI sites of pDEX777 for overexpressing proteins of your interest with/without GFP and with/without NLS. GFP is in between HindIII and XbaI. NLS from Tbrucei La protein (RGHKRSRE) is located in between AgeI and MfeI.

With NLS:
You can clone your gene of interest using MfeI/EcoRV/NdeI/PacI/AscI/BclI/PmeI/BamHI/AflII

Without NLS:
You can clone your gene of interest using XbaI/ClaI/AgeI and PmlI/MfeI/EcoRV/NdeI/PacI/AscI/BclI/PmeI/BamHI/AflII

Make sure your gene of interest is inserted in frame with GFP and/or NLS. Include start or stop codon as appropriate.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBA310 was a gift from Bungo Akiyoshi (Addgene plasmid # 190002 ; ; RRID:Addgene_190002)
  • For your References section:

    Targeted protein degradation using deGradFP in Trypanosoma brucei. Ishii M, Akiyoshi B. Wellcome Open Res. 2022 Jun 23;7:175. doi: 10.12688/wellcomeopenres.17964.1. eCollection 2022. 10.12688/wellcomeopenres.17964.1 PubMed 35865221