pAAV2-CAGIG-H2B-V5-mScarlet
(Plasmid #191093)

• Purpose: To express a bright monomeric red FP to label eucaryotic cell nuclei. To be used in tissue clearing methods and other fluorescent microscopy methods
• Depositing Labs: Murray Blackmore, Pantelis Tsoulfas
• Publication: Wang et al Elife. 2022 Jul 15;11. pii: 76254. doi: 10.7554/eLife.76254.

Backbone

• Vector backbone: pAAV2-CAGIG
• Backbone manufacturer: Original was from the Boyden laboratory
• Backbone size w/o insert (bp): 4718
• Total vector size (bp): 5861
• Modifications to backbone: The originalpAAV2-CAG backbone has been shortened and added more restriction enzymes to make it more modular and delete specific parts for substitutions with other pieces of DNA.
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H2B and mScarlet
• Species: Synthetic
• Insert Size (bp): 1143
• Mutation: The fusion protein was optimized to the Human codon usage
• GenBank ID: NP_001100822
• Promoter: CMV and CAG
• Tag / Fusion Protein:
  • H2B-V5-mScarlet (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRV (not destroyed)
• 5′ sequencing primer: pCAGG-5
• 3′ sequencing primer: WPRE-R

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry