AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS
(Plasmid
#191527)
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PurposeDonor for targeted integration of 3xFLAG-2xSTREP tagged S-phase specific probe (FUCCI-S) to the AAVS1 safe harbor locus
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Depositing Lab
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Depositing OrganizationUniversite Laval
Located in Canada -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 191527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 191527-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 191527-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneAAVS1_Puro_PGK1_3xFLAG_Twin_Strep (Addgene plasmid # 68375)
- Backbone size w/o insert (bp) 5737
- Total vector size (bp) 7270
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Vector typeMammalian Expression, CRISPR, Synthetic Biology
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehGEM(1-110)_mNG_hSLBP(18-108)
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Alt nameS-phase specific probe tagged with 3xFLAG-2xSTREP tag
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SpeciesH. sapiens (human), Synthetic
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Insert Size (bp)-6
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GenBank ID51053 7884
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Entrez GeneGMNN (a.k.a. Gem, MGORS6)
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Entrez GeneSLBP (a.k.a. HBP)
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Tag
/ Fusion Protein
- mNeonGreen (mNG) + 3xFLAG_TwinStrep (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
--Expression is driven by the human PGK1 promoter and BGH polyA
-hGEM(1-110) sequence is derived from the original FUCCI system PMID: 18267078
-Use in combination with eSpCas9(1.1)_No_FLAG_AAVS1_T2 (Plasmid #79888)
DNA (Catalog # 191527-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 191527-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAVS1_Puro_hPGK1_hGEM(1-110)_mNG_hSLBP(18-108)_FS was a gift from Yannick Doyon (Addgene plasmid # 191527 ; http://n2t.net/addgene:191527 ; RRID:Addgene_191527)
