eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid
(Plasmid
#192242)
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Purposeknockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3f
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Depositing Lab
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Depositing OrganizationXiamen University
Located in China -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192242 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 192242-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 192242-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCR-Blunt II-TOPO vector(Cat#450245)
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Backbone manufacturerThermo fisher
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeIF3f-knockin-homology arm-mAID-mCherry2-Hygro
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SpeciesH. sapiens (human)
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Insert Size (bp)5060
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Entrez GeneEIF3F (a.k.a. EIF3S5, MRT67, eIF3-p47)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CAGGAAACAGCTATGAC
- 3′ sequencing primer TGTAAAACGACGGCCAGT
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.08.28.505560v2 for bioRxiv preprint.
DNA (Catalog # 192242-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 192242-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
eIF3f- pMK293 (mAID-mCherry2-Hygro) plasmid was a gift from Dieter Wolf (Addgene plasmid # 192242 ; http://n2t.net/addgene:192242 ; RRID:Addgene_192242) -
For your References section:
eIF3 mRNA selectivity profiling reveals eIF3k as a cancer-relevant regulator of ribosome content. Duan H, Zhang S, Zarai Y, Ollinger R, Wu Y, Sun L, Hu C, He Y, Tian G, Rad R, Kong X, Cheng Y, Tuller T, Wolf DA. EMBO J. 2023 Jun 15;42(12):e112362. doi: 10.15252/embj.2022112362. Epub 2023 May 8. 10.15252/embj.2022112362 PubMed 37155573
