pEN_TT 3xFLAG-DYNLL2
(Plasmid
#192296)
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PurposeGateway entry vector for an inducible 3xFLAG-DYNLL2
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 192296 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEN_TTmiRC2
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Backbone manufacturerAddgene #25752
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Vector typeGateway entry vector
Growth in Bacteria
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Bacterial Resistance(s)Gentamicin, 10 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDYNLL2
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Alt nameDNCL1B
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Alt nameDlc2
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SpeciesH. sapiens (human)
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Entrez GeneDYNLL2 (a.k.a. DNCL1B, Dlc2, RSPH22)
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Tag
/ Fusion Protein
- 3xFLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SpeI (not destroyed)
- 3′ cloning site MfeI (not destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pEN_TT 3xFLAG-DYNLL2 was a gift from Kevin Janes (Addgene plasmid # 192296 ; http://n2t.net/addgene:192296 ; RRID:Addgene_192296) -
For your References section:
Nucleocytoplasmic transport of active HER2 causes fractional escape from the DCIS-like state. Wang L, Paudel BB, McKnight RA, Janes KA. Nat Commun. 2023 Apr 13;14(1):2110. doi: 10.1038/s41467-023-37914-x. 10.1038/s41467-023-37914-x PubMed 37055441