pCMV-PE2-tagRFP-BleoR
(Plasmid #192508)

• Purpose: Expression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.
• Depositing Lab: Gerald Schwank
• Publication: Mathis et al Nat Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01613-7.

Backbone

• Vector backbone: pCMV-PE2 (#132775)
• Backbone manufacturer: David Liu
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PE2-P2A-tagRFP-P2A-BleoR
• Alt name: Prime editor 2-P2A-tagRFP-P2A-BleoR
• Species: Synthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus
• Insert Size (bp): 7512
• Tags / Fusion Proteins:
  • SV40 bpNLS (N terminal on insert)
  • SV40 bpNLS (C terminal on insert)
  • tagRFP (C terminal on insert)
  • BleoR (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pCMV-PE2-tagRFP-BleoR.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry