pCMV-PE2-tagRFP-BleoR
(Plasmid
#192508)
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PurposeExpression of PE2 in mammalian cells with co-expression of tagRFP and Zeocin resistance gene.
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Depositing Lab
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Depositing OrganizationUniversity of Zurich
Located in Switzerland -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192508 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 192508-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 192508-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCMV-PE2 (#132775)
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Backbone manufacturerDavid Liu
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namePE2-P2A-tagRFP-P2A-BleoR
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Alt namePrime editor 2-P2A-tagRFP-P2A-BleoR
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SpeciesSynthetic; SpCas9 is from Streptococcus pyogenes; MMLV_RT is from the Moloney murine leukemia virus
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Insert Size (bp)7512
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Tags
/ Fusion Proteins
- SV40 bpNLS (N terminal on insert)
- SV40 bpNLS (C terminal on insert)
- tagRFP (C terminal on insert)
- BleoR (C terminal on insert)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 192508-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 192508-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-PE2-tagRFP-BleoR was a gift from Gerald Schwank (Addgene plasmid # 192508 ; http://n2t.net/addgene:192508 ; RRID:Addgene_192508) -
For your References section:
Predicting prime editing efficiency and product purity by deep learning. Mathis N, Allam A, Kissling L, Marquart KF, Schmidheini L, Solari C, Balazs Z, Krauthammer M, Schwank G. Nat Biotechnol. 2023 Jan 16. doi: 10.1038/s41587-022-01613-7. 10.1038/s41587-022-01613-7 PubMed 36646933
Map uploaded by the depositor.
