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pcDNA3.1_mGFP-FGFR3-mScarlet
(Plasmid #192509)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 192509 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 192509-D50 50 μg of DNA in Tris buffer $495
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DNA 192509-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone size w/o insert (bp) 5529
  • Total vector size (bp) 9429
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    mGFP-FGFR3IIIc-mScarlet
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3906
  • GenBank ID
    NM_000142.5
  • Entrez Gene
    FGFR3 (a.k.a. ACH, CD333, CEK2, HSFGFR3EX, JTK4)
  • Tags / Fusion Proteins
    • mGFP (N terminal on insert)
    • mScarlet (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV_F
  • 3′ sequencing primer pcDNA3.1_R
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1_mGFP-FGFR3-mScarlet was a gift from Irene Tiemann-Boege (Addgene plasmid # 192509 ; http://n2t.net/addgene:192509 ; RRID:Addgene_192509)

  • For your References section:

    Measurement of FGFR3 signaling at the cell membrane via total internal reflection fluorescence microscopy to compare the activation of FGFR3 mutants. Hartl I, Brumovska V, Striedner Y, Yasari A, Schutz GJ, Sevcsik E, Tiemann-Boege I. J Biol Chem. 2023 Feb;299(2):102832. doi: 10.1016/j.jbc.2022.102832. Epub 2022 Dec 27. 10.1016/j.jbc.2022.102832 PubMed 36581204
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