pILL5-NLS-3xmCitrine
(Plasmid #192525)

• Purpose: Fluorescent reporter for plant responses to bacterial infection
• Depositing Lab: Gitta Coaker
• Publication: Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi: 10.1016/j.celrep.2023.112676.

Backbone

• Vector backbone: pMpGWB123
• Backbone size w/o insert (bp): 13428
• Total vector size (bp): 13910
• Vector type: Plant Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Spectinomycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: IAA-leu-resistant-like5
• Alt name: ILL5
• Species: A. thaliana (mustard weed)
• Insert Size (bp): 2089
• GenBank ID: AT1G51780
• Entrez Gene: ILL5 (a.k.a. AT1G51780, F19C24.29, F19C24_29, IAA-leucine resistant (ILR)-like gene 5)
• Promoter: ILL5
• Tags / Fusion Proteins:
  • NLS (C terminal on insert)
  • 3xmCitrine (C terminal on backbone)

Cloning Information

• Cloning method: Gateway Cloning
• 5′ sequencing primer: TGTAAAACGACGGCCAGT

Resource Information

• Supplemental Documents:
  • pILL5-NLS-3xmCitrine.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry