pGBdest-10xHis-TEV-ATG5_ATG7_ATG10_ATG12_ATG16L1_F32A-I35A-I36A_-GFP-TEV-StrepII
(Plasmid #192705)

• Purpose: Plasmid for the Expression of the E3 complex with an FII (F32A, I35A, and I36A) mutation. SMC Internal reference: SMC1728
• Depositing Lab: Sascha Martens
• Publication: Sascha Martens Lab: ASAP Shared Plasmids (unpublished)

Backbone

• Vector backbone: pGBDest
• Backbone manufacturer: Vienna Biocenter Core Facilities GmbH, Vienna, Austria
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 13584
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: ATG5
• Alt name: APG5
• Species: H. sapiens (human)
• Insert Size (bp): 894
• GenBank ID: NC_000006.12
• Tag / Fusion Protein:
  • 10x-His-TEV (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATGCACCATCACCATCACCATCACCATCACCATG
• 3′ sequencing primer: CCCCAGAACATCAGGTTAATGGCGTTAGTCCGTAGGTTGG

Gene/Insert 2

• Gene/Insert name: ATG7
• Species: H. sapiens (human)
• Insert Size (bp): 2112
• GenBank ID: NC_000003.12

Cloning Information for Gene/Insert 2

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATGGCAGCGGCTACTGGTGACCCTGGCTTG
• 3′ sequencing primer: TTAAATAGTCTCATCGTCGCTCATATCCCA

Gene/Insert 3

• Gene/Insert name: ATG10
• Species: H. sapiens (human)
• Insert Size (bp): 663
• GenBank ID: NC_000005.10

Cloning Information for Gene/Insert 3

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATGGAGGAGGACGAGTTCATCGGAGAAAAG
• 3′ sequencing primer: TTAGGGCACGTTTCTTTCGTCTTGGC

Gene/Insert 4

• Gene/Insert name: ATG12
• Species: H. sapiens (human)
• Insert Size (bp): 423
• GenBank ID: NC_000005.10

Cloning Information for Gene/Insert 4

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATGGCCGAAGAGCCCCAGAGCGTTT
• 3′ sequencing primer: TTAGCCCCACGCTTGTGATTTGCAGTAATG

Gene/Insert 5

• Gene/Insert name: ATG16L1
• Insert Size (bp): 2631
• Mutation: F32A-I35A-I36A
• GenBank ID: NC_000002.12
• Tag / Fusion Protein:
  • -GFP-TEV-StrepII (C terminal on insert)

Cloning Information for Gene/Insert 5

• Cloning method: Gateway Cloning
• 5′ sequencing primer: ATGAGCAGCGGTTTGAGGGCGGCGGATTTC
• 3′ sequencing primer: TTACTTCTCGAACTGCGGATGCGACCAGGCC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry