pDONR221-HMGN1-SE
(Plasmid
#192720)
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PurposeGateway entry vector encoding mutant HMGN1
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192720 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepDONR221
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Backbone manufacturerInvitrogen
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Vector typeGateway Entry Vector
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHMGN1
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SpeciesH. sapiens (human)
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Insert Size (bp)303
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MutationS21E; S25E
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Entrez GeneHMGN1 (a.k.a. HMG14)
- Promoter None
Cloning Information
- Cloning method Gateway Cloning
- 5′ sequencing primer M13 forward
- 3′ sequencing primer M13 reverse (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMutagenized from pDONR221-HMGN1 plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
cDNA encodes NP_004956.5 with S21E and S25E mutations
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pDONR221-HMGN1-SE was a gift from Summer Thyme (Addgene plasmid # 192720 ; http://n2t.net/addgene:192720 ; RRID:Addgene_192720)
