pGK-hMIGA2-DsRed
(Plasmid
#192867)
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PurposeExpress codon-optimized human MIGA2 full length with a C-terminal DsRed tag in mammalian cells
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Depositing Lab
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Depositing OrganizationYale University
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192867 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 192867-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 192867-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonePlenti-PGK
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCodon-optimized human Mitoguardin 2
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Alt nameMIGA2 (C9orf54, FAM73B)
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SpeciesH. sapiens (human)
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Insert Size (bp)1779
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Entrez GeneMIGA2 (a.k.a. C9orf54, FAM73B)
- Promoter PGK
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Tag
/ Fusion Protein
- DsRed (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ggtggcctgcaggtgaattcATGGCGTTTCGTCGTGCG
- 3′ sequencing primer tatgggtactcgagttaCTGGGAGCCGGAGTGGCG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 192867-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 192867-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGK-hMIGA2-DsRed was a gift from Karin Reinisch (Addgene plasmid # 192867 ; http://n2t.net/addgene:192867 ; RRID:Addgene_192867) -
For your References section:
Mitoguardin-2-mediated lipid transfer preserves mitochondrial morphology and lipid droplet formation. Hong Z, Adlakha J, Wan N, Guinn E, Giska F, Gupta K, Melia TJ, Reinisch KM. J Cell Biol. 2022 Dec 5;221(12):e202207022. doi: 10.1083/jcb.202207022. Epub 2022 Oct 25. 10.1083/jcb.202207022 PubMed 36282247
