HyWnt3-ΔRep:Luc
(Plasmid
#192987)
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PurposePlasmid to test the Hydra Wnt3 promoter activity (full deletion of the repressor element)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192987 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGL3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameHydra Wnt3 promoter
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SpeciesHydra
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Insert Size (bp)1763
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MutationRepressor element deleted
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HyWnt3-ΔRep:Luc was a gift from Brigitte Galliot (Addgene plasmid # 192987 ; http://n2t.net/addgene:192987 ; RRID:Addgene_192987) -
For your References section:
An evolutionarily-conserved Wnt3/beta-catenin/Sp5 feedback loop restricts head organizer activity in Hydra. Vogg MC, Beccari L, Iglesias Olle L, Rampon C, Vriz S, Perruchoud C, Wenger Y, Galliot B. Nat Commun. 2019 Jan 18;10(1):312. doi: 10.1038/s41467-018-08242-2. 10.1038/s41467-018-08242-2 PubMed 30659200
