AAV-PGK-Chst3
(Plasmid #193024)

• Purpose: AAV vector plasmid expressing mouse chondroitin 6-sulfotransferase (Chst3) under the mouse phosphoglycerate kinase (PGK) promoter
• Depositing Lab: Bart Nieuwenhuis
• Publication: Yang et al Mol Psychiatry. 2021 Jul 16. pii: 10.1038/s41380-021-01208-9. doi: 10.1038/s41380-021-01208-9.

Backbone

• Vector backbone: Addgene plasmid #24593
• Backbone manufacturer: Patrick Aebischer
• Total vector size (bp): 6054
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Chst3
• Species: M. musculus (mouse)
• Insert Size (bp): 1512
• Entrez Gene: Chst3 (a.k.a. C6ST, C6ST-1, GST-0)
• Promoter: PGK
• Tags / Fusion Proteins:
  • myc-tag (C terminal on insert)
  • 3xFLAG (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: AfeI (destroyed during cloning)
• 5′ sequencing primer: Not applicable
• 3′ sequencing primer: Not applicable

Resource Information

• Supplemental Documents:
  • AAV-PGK-Chst3.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry