Perox-mTurq2-Apollo-NADP+
(Plasmid #193304)

• Purpose: Expresses mTurq2-Apollo-NADP+ sensor localized to the peroxisome
• Depositing Lab: Jonathan Rocheleau
• Depositing Organization: University of Toronto
  Located in Canada
• Publication: Chang et al ACS Sens. 2022 Oct 21. doi: 10.1021/acssensors.2c01174.

Backbone

• Vector backbone: pcDNA3.1(+)
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: G6PD
• Alt name: glucose-6-phosphate dehydrogenase
• Species: H. sapiens (human)
• Insert Size (bp): 2736
• Mutation: S40A, R72Q, H201N, K205T
• Entrez Gene: G6PD (a.k.a. CNSHA1, G6PD1)
• Promoter: T7
• Tag / Fusion Protein:
  • mTurqoise2, peroxisomal targeting sequence (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (mTurq2) (unknown if destroyed)
• 3′ cloning site: XhoI/XbaI (mTurq2) (unknown if destroyed)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: G6PD cloned from vector purchased from DNASU. Clone ID: FLH023345.01L

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Contains a C-terminal mTurqoise2 tag. Contains a C-terminal -SKL peroxisomal targeting sequence.

Please visit https://doi.org/10.1101/2022.04.27.489691 for bioRxiv preprint.

DNA (Catalog # 193304-D50)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 50 μg
• Concentration: 1 μg/μL
• Pricing: $495 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

DNA (Catalog # 193304-D100)

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

• Amount: 100 μg
• Concentration: 1 μg/μL
• Pricing: $585 USD
• Storage: 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.