pcDNA5/FRT/TO
(Plasmid
#19361)
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Purpose(Empty Backbone)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 19361 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepcDNA5/FRT/TO
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Backbone manufacturerInvitrogen
- Backbone size (bp) 5137
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer LNCX
- 3′ sequencing primer BGH-rev
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA5/FRT/TO was a gift from Harm Kampinga (Addgene plasmid # 19361) -
For your References section:
Computational analysis of the human HSPH/HSPA/DNAJ family and cloning of a human HSPH/HSPA/DNAJ expression library. Hageman J, Kampinga HH. Cell Stress Chaperones. 2009 Jan;14(1):1-21. doi: 10.1007/s12192-008-0060-2. Epub 2008 Aug 7. 10.1007/s12192-008-0060-2 PubMed 18686016