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pEGFP-Scribble
(Plasmid #19376)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 19376 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    CLONTECH Laboratories, Inc.
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418) ; G418

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    DH5α, HB101, and other general purpose strains. Single-stranded DNA production requires an F plasmid host such as JM109 or XL1-Blue.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Scribble
  • Alt name
    hScrib
  • Alt name
    Scrib
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4907
  • Mutation
    Full length.
  • GenBank ID
    NM_182706
  • Entrez Gene
    SCRIB (a.k.a. CRIB1, SCRB1, SCRIB1, Vartul, oSCRIB)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-N Sequencing Primer (#6479-1): 679–658
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Partial cDNA to human Scrib was provided by J. Huibregtse (University of Texas, Austin, TX).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-Scribble was a gift from Ian Macara (Addgene plasmid # 19376)

  • For your References section:

    The mammalian Scribble polarity protein regulates epithelial cell adhesion and migration through E-cadherin. Qin Y, Capaldo C, Gumbiner BM, Macara IG. J Cell Biol. 2005 Dec 19. 171(6):1061-71. 10.1083/jcb.200506094 PubMed 16344308
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