pPINA7A2mTFP1(F)
(Plasmid
#193774)
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PurposeExpresses mTFP1 under the control of PINA7 synthetic promoter, ColE1 origin of replication, Ampicillin selection
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 193774 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepZ
- Backbone size w/o insert (bp) 2430
- Total vector size (bp) 3141
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Vector typeBacterial Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemTFP1
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SpeciesBacteria
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Insert Size (bp)711
- Promoter PINA7 synthetic promoter carrying binding sites for lambda CI and LacI
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site XbaI (unknown if destroyed)
- 5′ sequencing primer GAGACCACAATGGGCGTAAT
- 3′ sequencing primer CGTAAACGGTCACCTTGTTGTA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPINA7A2mTFP1(F) was a gift from Sangram Bagh (Addgene plasmid # 193774 ; http://n2t.net/addgene:193774 ; RRID:Addgene_193774) -
For your References section:
A single layer artificial neural network type architecture with molecular engineered bacteria for reversible and irreversible computing. Sarkar K, Bonnerjee D, Srivastava R, Bagh S. Chem Sci. 2021 Nov 9;12(48):15821-15832. doi: 10.1039/d1sc01505b. eCollection 2021 Dec 15. 10.1039/d1sc01505b PubMed 35024106
Map uploaded by the depositor.
