pRA1SEGFPTcIfm
(Plasmid #193777)

• Purpose: Cassette 1: Expresses EGFP under the control of PR promoter, Cassette 2: Expresses frame-shifted CI under the control of PLTetO-1 promoter, pUC origin of replication, Ampicillin selection
• Depositing Lab: Sangram Bagh
• Publication: Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.1186/s13036-019-0151-x. eCollection 2019.

Backbone

• Vector backbone: pZ
• Backbone size w/o insert (bp): 2367
• Total vector size (bp): 4479
• Vector type: Bacterial Expression, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: EGFP and frame-shifted CI in opposite orientation, controlled by separate promoters and terminators
• Species: Bacteria
• Insert Size (bp): 2112
• Promoter: PR promoter and PLTetO-1

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AvrII (unknown if destroyed)
• 3′ cloning site: AvrII (unknown if destroyed)
• 5′ sequencing primer: GTTTTGGAGCACG GAAAGAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry