Tet-inducible 3xFlag Mouse C325A Caspase-9 ΔCARD
(Plasmid #194608)

• Purpose: Inducible Expression of N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus CARD Domain
• Depositing Lab: Hannah Rabinowich
• Publication: Han et al J Biol Chem. 2014 Mar 7;289(10):6485-6497. doi: 10.1074/jbc.M113.536854. Epub 2013 Dec 20.

Backbone

• Vector backbone: pcDNA4/TO
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5078
• Total vector size (bp): 5994
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: N-Terminal 3X Flag Mouse C325A Caspase-9 Mutant Minus Sequence Encoding Met 1 to Lys 176
• Alt name: CASP-9; ICE-LAP6; Mch6; APAF-3
• Species: M. musculus (mouse)
• Insert Size (bp): 945
• Mutation: Deletion of N-terminal CARD domain and changed Cysteine 325 to Alanine to eliminate protease activity
• GenBank ID: NM_015733.5
• Entrez Gene: Casp9 (a.k.a. APAF-3, CASP-9, Caspase-9, ICE-LAP6, Mch6)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3X Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CTGGAGACGCCATCCACG
• 3′ sequencing primer: TAGAAGGCACAGTCGAGG

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry