pgRNA_hMECP2_promoter_No8
(Plasmid #194902)

• Purpose: Lentiviral construct with mCherry and Puro cassettes to express sgRNA-8 targeting the promoter of human MECP2.
• Depositing Lab: Shawn Liu
• Publication: Qian et al Sci Transl Med. 2023 Jan 18;15(679):eadd4666. doi: 10.1126/scitranslmed.add4666. Epub 2023 Jan 18.

Backbone

• Vector backbone: pgRNA-modified
• Backbone manufacturer: Addgene 84477
• Total vector size (bp): 8319
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: sgRNA targeting human MECP2 promoter No.8
• Species: Synthetic
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AarI (destroyed during cloning)
• 3′ cloning site: AarI (destroyed during cloning)
• 5′ sequencing primer: gaaactcaccctaactg

Resource Information

• Supplemental Documents:
  • pgRNA_hMECP2_promoter_No8.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

contain a CMV-Puro-T2A-mCherry cassette allowing for FACS isolation or puromycin selection.