PB-Blast-Dest-LifeAct-mRuby
(Plasmid #194907)

• Purpose: To generate LifeAct-mRuby expressing stable cell lines with piggybac transposase co-expression and blastocidin selections.
• Depositing Lab: Clare Buckley
• Publication: Liang et al EMBO J. 2022 Aug 22:e111021. doi: 10.15252/embj.2022111021.

Backbone

• Vector backbone: PB/Blast-Dest
• Backbone size w/o insert (bp): 7100
• Total vector size (bp): 7939
• Vector type: Mammalian Expression, Mouse Targeting
• Selectable markers: Blasticidin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: LifeAct
• Insert Size (bp): 51
• Tag / Fusion Protein:
  • mRuby (C terminal on insert)

Resource Information

• Supplemental Documents:
  • EMBO J plasmid PB-Blast-Dest-LifeAct-mRuby.gbk
• A portion of this plasmid was derived from a plasmid made by: LifeAct cDNA was taken from the plasmid published in Meng et al 2017: https://doi.org/10.1038/s41467-017-00977-8

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry