lenti-fullef1a-FAKSPARK2
(Plasmid
#195265)
-
Purposeexpress wtFAKSPARK2 in a lenti vector
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 195265 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonelenti vector
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameFAKSPARK2 sensor
-
Insert Size (bp)2356
- Promoter ef1a
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site AgeI (destroyed during cloning)
- 3′ cloning site EcoRI (unknown if destroyed)
- 5′ sequencing primer ef1aF
- 3′ sequencing primer wprer (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
lenti-fullef1a-FAKSPARK2 was a gift from Xiaokun Shu (Addgene plasmid # 195265 ; http://n2t.net/addgene:195265 ; RRID:Addgene_195265) -
For your References section:
Polarized focal adhesion kinase activity within a focal adhesion during cell migration. Li X, Combs JD 3rd, Salaita K, Shu X. Nat Chem Biol. 2023 Jun 22. doi: 10.1038/s41589-023-01353-y. 10.1038/s41589-023-01353-y PubMed 37349581
