Halo-TMD
(Plasmid
#195347)
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PurposeFluorescent labeling for cell membranes
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Depositing Lab
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Depositing OrganizationUniversity of Michigan
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 195347 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 195347-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 195347-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneTfR-sfGFP-myc tag-SpyCatcher003 Sequences
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Backbone manufacturerAddgene
- Backbone size w/o insert (bp) 5810
- Total vector size (bp) 6698
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Vector typeMammalian Expression
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Selectable markersZeocin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHaloTag
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SpeciesSynthetic
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Insert Size (bp)888
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ATCGGCACGGGCTTTCCGTTTG
- 3′ sequencing primer TTCCAGCCCGGAGATCTCCAGTG
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.10.04.510821v2 for bioRxiv preprint.
DNA (Catalog # 195347-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 195347-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Halo-TMD was a gift from Allen Liu (Addgene plasmid # 195347 ; http://n2t.net/addgene:195347 ; RRID:Addgene_195347) -
For your References section:
Mechanosensitive Channel-Based Optical Membrane Tension Reporter. Hsu YY, Resto Irizarry AM, Fu J, Liu AP. ACS Sens. 2023 Jan 27;8(1):12-18. doi: 10.1021/acssensors.2c01921. Epub 2023 Jan 6. 10.1021/acssensors.2c01921 PubMed 36608338
Map uploaded by the depositor.
